Particle Counter

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Analyzing…

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Particles are

Fluorescence: bright. Brightfield/stained: usually dark.

Radius (px)20

Set larger than the radius of your biggest particle. 0 = off.

Smoothing

Automatic (Otsu)

Level128

Check the Mask view: particles should be white, background black.

Min area (px)

Max area (px)

Exclude particles touching the edge

µm per pixel

Scale lets areas report in µm². Find it in your microscope's metadata.

Drag on the image to draw. Particles are counted in the ROI by their center.

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